One of the most important factors that creates the impression of an organization is business communication. Business communication can sometimes be a crucial factor between success and failure. Many times, a misunderstanding or insufficient quality of business communication was the cause of not concluding a deal or poor business performance. Employees of a certain company in business communication represent themselves, but also the company or organization in which they are employed. The first impression, which takes 7 to 30 seconds to create, leaves the deepest trace and is remembered for a long time, mostly for the simple reason that it is the first. People create first impressions instinctively and on an unconscious, emotional level, which speaks in favor of emotions playing a significant role in gaining first impressions, as well as in business communication. Factors that influence and are part of creating a first impression of a person are: body language, physical appearance, verbal communication, dress and mood, stereotypes. During the first meeting, ie in the first few moments, people assess whether they can have respect for a certain person and whether it is possible to trust the person with whom they are in current communication. A survey was conducted through a questionnaire on the impact of first impressions on business communication and the results showed that first impressions have an extremely large impact on business communication.