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master's thesis
Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2014. urn:nbn:hr:109:911356
Ćurković, Ivana
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Process Design and Construction Materials

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Ćurković, I. (2014). Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:911356

Ćurković, Ivana. "Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2014. https://urn.nsk.hr/urn:nbn:hr:109:911356

Ćurković, Ivana. "Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2014. https://urn.nsk.hr/urn:nbn:hr:109:911356

Ćurković, I. (2014). 'Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:109:911356

Ćurković I. Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2014 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:109:911356

I. Ćurković, "Projektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:109:911356

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Metadata
TitleProjektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima
Title (english)Design of a bioreactor for microorganism cultivation in semi-solid and solid substrates
AuthorIvana Ćurković
MentorDarko Velić (mentor)
MentorMarina Tišma (komentor)
Committee memberNatalija Velić
Committee memberDarko Velić
Committee memberMarina Tišma
Committee memberMirela Planinić
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Process Design and Construction Materials)
Osijek
Defense date and country2014-04-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Fermentacije na čvrstim nosačima (eng. Solid State Fermentations, SSF) su procesi koji podrazumijevaju rast mikroorganizama na vlažnim čvrstim nosačima bez prisustva slobodne vode, pri čemu čvrsti nosači mogu ujedno biti i supstrati za rast, tzv. neinertni nosači. Jedan od primjera neinertnih nosača u SSF su različiti lignocelulozni materijali, pri čemu se SSF primjenjuje za mikrobiološku razgradnju ovih materijala. Lignocelulozni materijali predstavljaju važan obnovljivi izvor energije i kao otpad u velikim količinama nastaju u poljoprivrednoj, prehrambenoj i drvnoj industriji. Nadalje, ovi materijali zanimljivi su i kao potencijalna sirovina u biotehnološkoj proizvodnji, pri čemu najveći problem predstavlja teška razgradnja lignoceluloznih sastavnica (celuloze, hemiceluloze i lignina) do jednostavnih šećera. Fermentacije na čvrstim nosačima uspješno se primjenjuju za mikrobiološku razgradnju lignocelulozne biomase, pri čemu se kao radni mikroorganizam najčešeće koriste gljive bijelog truljenja koje imaju sposobnost razgradnje lignoceluloznih sastavnica do fermentabilnih šećera. U ovom radu, gljiva bijelog truljenja, Trametes versicolor uzgajana je na repinim rezancima kao čvrstom supstratu i nosaču u reaktorskom sustavu pri ambijentalnoj temperaturi tijekom 30 dana. Istraživan je utjecaj T. versicolor na razgradnju repinih rezanaca. Tijekom fermentacije praćene su promjene procesnih parametra (temperatura i relativna vlažnost u sustavu), kao i gubitak na masi lignoceluloznog supstrata te promjena boje i kemijskog sastava supstrata. Povećanje temperature i relativne vlažnosti u sustavu, kao i gubitak na masi supstrata od 21,33 % ukazuju na intenzivan rast i aktivnost radnog mikroorganizma. Nadalje, konverzija celuloze od 64,65 % i pentozana od 42,39 % nakon 30 dana uzgoja također ukazuju na aktivnost radnog mikroorganizma i produkciju enzima odgovornih za razgradnju lignoceluloznih sastavnica. Na osnovi provedenog eksperimenta i dobivenih rezultata projektiran je horizontalni bioreaktor s kombiniranim sustavom miješanja.
Abstract (english)
Solid State Fermentation (SSF) is a process where the microorganisms are grown on moist solid substrates in the absence of free water, where the solid carrier can also be a substrate for the microorganism growth, so-called, non-reactive substrate. An example of non-reactive substrates in SSF are different lignocellulosic materials, where in the SSF is applied for the microbiological decomposition of these materials. Lignocellulosic materials represent an important renewable energy source occurring as waste in large quantities in the agricultural, food and timber industries. These materials, however,could have application in biotechnological production, but the biggest problem is the severe degradation of lignocellulosic components (cellulose, hemicellulose and lignin) to simple sugars. SSF is successfully applied for microbial degradation of the lignocellulose, where in white rot fungi are the most often used microorganisms. In this study white rot fungi, Trametes versicolor was cultivated on sugar beet pulp as a solid substrate and the carrier in the reactor at ambient temperature for 30 days. The possibility of T. versicolor to degrade beet pulp was investigated. During fermentation, the change of process parameters was observed (temperature and relative humidity in the system), as well as the loss of substrate mass,change of the color and chemical composition. Temperature and relative humidity in crease as well as the loss of substrate mass (21,33%) indicate strong growth and the activity of working microorganisms. Furthermore, the conversion of cellulose 64.65% and 42.39% of pentosans after 30 days of cultivation also indicates the activity of working microorganism and production of enzymes responsible for degradation of lignocellulosic components. A horizontal bioreactor with combined interference system was designed based on obtained results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:911356
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology Osijek
Created on2015-09-24 13:13:02