master's thesis
DETERMINATION GLIADIN LOCI OF CROATIAN WINTER WHEAT GERMPLASM

Marija Grgić (2015)
Metadata
TitleZASTUPLJENOST GLIJADINSKIH LOKUSA GERMPLAZME HRVATSKE OZIME PŠENICE
AuthorMarija Grgić
Mentor(s)Sonja Petrović (thesis advisor)
Abstract
Cilj ovog istraživanja bio je ispitati i utvrditi zastupljenost glijadinskih lokusa u dvadeset hrvatskih sorata ozime pšenice. Za analizu i identifikaciju podjedinica na Gli-B1 i Gli-D1 lokusima korištena je poliakrilamid gel elektroforeza (PAGE) u prisutnosti natijeva dodecil sulfata (SDS). Na Gli-B1 lokusima najvecu zastupljenost su imale kombinacije podjedinica 63+67 s frekvencijom od 50%, dok je najmanju zastupljenost imala podjedinica 61 s frekvencijom od 5%. Prisutne su bile i podjedinice 60, 66 i nulti alel (N). Na Gli-D1 lokusu najzastupljenija je bila podjedinica 55 s frekvencijom od 90%, prisutna je bila kombinacija podjedinica 55+56+59 s frekvencijom od 5%, te podjedinica 59 s frekvencijom takoer od 5%.
Keywordswheat -gliadins subunits SDS PAGE
Parallel title (English)DETERMINATION GLIADIN LOCI OF CROATIAN WINTER WHEAT GERMPLASM
Committee MembersSonja Marić
Ivica Strelec
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Poljoprivredni fakultet
Lower level organizational unitsZavod za Bilinogojstvo
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Agronomy
Seed Production
Study programme typeuniversity
Study levelgraduate
Study programmePlant Production, specialization: Plant Production, Plant Nutrition and Soil Science, Plant Selection and Seed Production, Plant Protection
Academic title abbreviationmag. ing. agr.
Genremaster's thesis
Language Croatian
Defense date2015-06-19
Parallel abstract (English)
The aim of this study was to examine and determine distibution of Gli-B1 and Gli-D1 loci in twenty Croatian winter wheat varieties using SDS PAGE. At Gli-B1 loci we determined prevalence of subunits 63+67 combination with a frequency of 50%, while the lowest prevalence had subunit 61 with a frequency of 5%. Subunits 60, 66 and null allele (N) were also present. At Gli-D1 locus, the most common subunit was 55 with a frequency of 90%, combination of subunits 55 + 56 + 59 and the subunit 59 were also present with frequency of 5%.
Parallel keywords (Croatian)pšenica -glijadini podjedinica SDS PAGE
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Notediplomski rad
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:151:552730
CommitterLjiljana Vučković Vizentaner