Izolacija genomske DNA iz zrna pšenice

Stilin, Anamarija

undergraduate thesis

Izolacija genomske DNA iz zrna pšenice

Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2019. urn:nbn:hr:151:129560

Stilin, Anamarija

Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Cite this document

APA 6th Edition

Stilin, A. (2019). Izolacija genomske DNA iz zrna pšenice (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:129560

MLA 8th Edition

Stilin, Anamarija. "Izolacija genomske DNA iz zrna pšenice." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:151:129560

Chicago 17th Edition

Harvard

Stilin, A. (2019). 'Izolacija genomske DNA iz zrna pšenice', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:151:129560

Vancouver

Stilin A. Izolacija genomske DNA iz zrna pšenice [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2019 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:151:129560

IEEE

A. Stilin, "Izolacija genomske DNA iz zrna pšenice", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:151:129560

Cite this item: https://urn.nsk.hr/urn:nbn:hr:151:129560

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Metadata

Title	Izolacija genomske DNA iz zrna pšenice
Title (english)	Genomic DNA extraction from wheat grain
Author	Anamarija Stilin
Mentor	Sonja Petrović (mentor)
Committee member	Sonja Vila (predsjednik povjerenstva)
Committee member	Andrijana Rebekić (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Faculty of Agrobiotechnical Sciences Osijek (Department of Plant Production and Biotechnology) Osijek
Defense date and country	2019-09-24, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy Genetics and Breeding of Plants, Animals and Microorganisms
Abstract	Pšenica je iza kukuruza druga najznačajnija žitarica iz porodice trava. Cilj ovog rada bio je uspješno izolirati genomsku DNA iz mljevenog zrna pšenice bez primjesa. Samo istraživanje provelo se u dvije faze: u prvoj fazi provedene su dvije metode izolacije DNA iz zrna pšenice, a to su modificirana CTAB metoda s dodatkom PVP i SDS metoda, a DNeasy® Plant Mini Kit test korišten je kao brza provjera kvalitete DNA . Druga faza sastoji se od spektrofotometrijske analize izolirane DNA. Modificirana CTAB metoda se pokazala kao najuspješnija s optimalnom čistoćom i koncentracijom izolirane DNA (A260/280 2,00 – 1,99, A260/230 2,19 – 2,34, koncentracija 97,10 – 181 ng/μL).
Abstract (english)	The wheat besides corn is the world the second most important crop of the grass family. The aim of this work was to successfully isolate genomic DNA from milled wheat grains without secondary methabolutes. The research was carried out in two phases: in the first phase were used two DNA isolation methods: modified CTAB method with PVP supplement and SDS method, and the DNeasy® Plant Mini Kit test was used as a quick DNA quality check. Second phase consist of spectrophotometric determination isolated DNA. Modified CTAB method was most successful generating optimal DNA quality and quantity results (A260/280 2.00 – 1.99, A260/230 2.19 – 2.34, concentration 97.10 – 181 ng/μL).
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:151:129560
Study programme	Title: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture Course: Horticulture Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Public note	završni rad
Repository	Repository of the Faculty of Agrobiotechnical Sciences Osijek
Created on	2019-09-30 08:45:14