master's thesis
Resistance of bacterial strains Klebsiella pneumoniae and Enterobacter cloacae isolated from urine samples to beta-lactam antibiotics

Marina Dujmić Ilić (2015)
Metadata
TitleRezistencija bakterijskih sojeva Klebsiella pneumoniae i Enterobacter cloacae izoliranih iz uzoraka urina na beta-laktamske antibiotike
AuthorMarina Dujmić Ilić
Mentor(s)Maja Miškulin (thesis advisor)
Abstract
Pozadina istraživanja: Sojevi K. pneumoniae i E. cloacae pokazuju različite vrste rezistencije β-laktamske antibiotike Ciljevi istraživanja: 1. utvrditi učestalost različitih vrsta rezistencije bakterijskih sojeva K. pneumoniae i E. cloacae na β-laktamske antibiotike u uzrocima urina pacijenata s područja Brodsko-posavske županije pomoću disk difuzije, određivanjem MIK-a ili uz pomoć fenotipizacije 2. procijeniti postoji li razlika u učestalosti pojave rezistencije između bolničkih i izvanbolničkih pacijenata. Nacrt studije: Presječna studija. Ispitanici i metode: U istraživanju je mikrobiološki obrađeno 100 uzoraka urina bolničkih i izvanbolničkih pacijenata koji su se javili u Službu za Kliničku mikrobiologiju Zavoda za javno zdravstvo Brodsko-posavske županije u Slavonskom Brodu. U uzorcima su detektirani bakterijski sojevi K. pneumoniae ili E. cloacae. Svi uzorci su testirani na rezistenciju na cefoskitin metodom disk difuzije te Double disk testom na produkciju ESBL, dok se fenotipizacijom detektirala produkcija AmpC β-laktamaza i karbapenemaza. Rezultati: Dobiveni rezultati pokazuju da je među bakterijskim sojevima Klebsiella pneumoniae i Enterobacter cloacae prisutna visoka učestalost rezistencije na cefoksitin, kao i produkcija ESBL i AmpC β-laktamaza. U istraživanju nije utvrđena produkcija karbapenemaza. Zaključak: U svijetu pa tako i u Hrvatskoj, zabilježen je porast rezistencije bakterijskih sojeva K. pneumoniae i E. cloacae na β-laktamske antibiotike. Pri tom je porast rezistencije utvrđen i u Brodsko-posavskoj županiji. Među navedenim sojevima otkrivena je visoka rezistencija na cefoksitin, visoka učestalost produkcije ESBL i AmpC β-laktamaza, dok nije utvrđena produkcija karbapenemaza. Otkriveno je da između bolničkih i izvanbolničkih pacijenata postoji statistički značajna razlika u rezistenciji na cefoksitin i u produkciji AmpC β-laktamaza kod bakterijskih sojeva K. pneumoniae i E. cloacae.
Keywordsβ-lactam antibiotics Enterobacter cloacae Klebsiella pneumoniae resistance
Parallel title (English)Resistance of bacterial strains Klebsiella pneumoniae and Enterobacter cloacae isolated from urine samples to beta-lactam antibiotics
Committee MembersJosip Milas (committee member)
Domagoj Drenjančević (committee member)
Maja Miškulin (committee member)
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Medicinski fakultet
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Study programme typeuniversity
Study levelgraduate
Study programmeUniversity graduate study; specializations in: medical laboratory diagnostics
Academic title abbreviationmag. med. lab. diag.
Genremaster's thesis
Language Croatian
Defense date2015-10-12
Parallel abstract (English)
The resistance of bacterial strains of Klebsiella pneumoniae and Enterobacter cloacae isolated from urine samples to β-lactam antibiotics Research background: Strains of K. pneumoniae and E. cloacae show different types of resistance to β-lactam antibiotics Research objectives: 1. to determine the frequency of different types of resistance of bacterial strains K. pneumoniae and E. cloacae to β-lactam antibiotics in the urine samples of the patients from the area of Brod-Posavina County by disk diffusion, determinig of MIK or with the help of phenotyping 2. to assess whether there is a difference in the frequency of resistance among hospital patients and outpatients. Study Plan: A cross-sectional study. Examinees and methods: In this study, 100 urine samples of hospital patients and outpatients, who applied to the Department for clinical microbiology of the Institute of Public Health of Brod-Posavina County in Slavonski Brod, were microbiologibcally processed. Bacterial strains of K. pneumoniae or E. cloacae were detected in the samples. All samples were tested for resistance to cefoxitin by disc diffusion method and by Double disk test for ESBL production, while production of AmpC β-lactamases and carbapenemases was detected by phenotyping. Results: The results show that among the bacterial strains Klebsiella pneumoniae and Enterobacter cloacae, a high frequency of resistance to cefoxitin, as well as the production of ESBLs and AmpC β-lactamase is present. The production of carbapemanases has not been determined. Conclusion: Worldwide, including Croatia, there is increasing resistance to bacterial strains of K. pneumoniae and E. cloacae to β-lactam antibiotics. Thereby, the increase in resistance was also registered in Brod-Posavina County. Among these strains, a high resistance to cefoxitin, high frequency of ESBL and AmpC β-lactamases production was detected, while the production of carbapenemases has not been determined. It was found that there is statistically significant difference in resistance to cefoxitin and in production of AmpC β-lactamases in bacterial strains of K. pneumoniae and E. cloacae between hospital patients and outpatients.
Parallel keywords (Croatian)β-laktamski antibiotici Enterobacter cloacae Klebsiella pneumoniae rezistencija
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:152:589602
CommitterBisera Kopf