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master's thesis
Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine, 2018. urn:nbn:hr:152:576015
Matić, Matea
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Matić, M. (2018). Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:576015

Matić, Matea. "Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:576015

Matić, Matea. "Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:576015

Matić, M. (2018). 'Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 01 April 2023, https://urn.nsk.hr/urn:nbn:hr:152:576015

Matić M. Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2023 April 01] Available at: https://urn.nsk.hr/urn:nbn:hr:152:576015

M. Matić, "Utjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:576015

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TitleUtjecaj novosintetiziranih derivata 6,7-dihidroksikumarina na rast stanica leukemija i limfoma in vitro
Title (english)Effects of new derivatives of 6,7-dihdroxycumarines on the leukemia and lymphoma cells' growth in vitro
AuthorMatea Matić
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberTeuta Opačak-Bernardi (predsjednik povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-12-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Maligne bolesti jedan su od glavnih uzroka smrtnosti u svijetu, a njihova se stopa pojavnosti u populaciji sve više povećava. Kumarini su spojevi koji imaju inhibicijski učinak na određene tumorske stanice te upravo zbog tih svojih antitumorskih obilježja imaju potencijalnu primjenu u terapiji malignih bolesti. Ciljevi istraživanja: Ispitati utjecaj derivata kumarina na rast normalnih stanica i stanica limfoma i leukemija in vitro. Odrediti promjene u staničnom ciklusu i način umiranja stanica. Materijali i metode: Inhibitorni učinak derivata kumarina (NS-48, NS-51 i A-153) određen je MTT testom na komercijalno dostupnim linijama humanih tumorskih stanica (CCFR, K562, Raji i HuT-78) i normalnoj staničnoj liniji (MDCK1). Promjene staničnog ciklusa i apoptoze praćene su na staničnoj liniji K562 i Raji protočnim citometrom. Rezultati:Testirani derivati kumarina pokazuju antiproliferativan i citotoksičan učinak na humane tumorske stanice. Inhibicija ovisi o koncentraciji spoja i staničnoj liniji. Na temelju rezultata može se zaključiti kako sva tri derivata kumarina djeluju inhibitorno na rast stanica pri koncentraciji od 1x10-4 M. Zaključak:Derivati kumarina u ovisnosti prema koncentracijama i tipu stanica djeluju inhibitorno na rast stanica.Uočljive su i promjene staničnog ciklusa te apoptoze.
Abstract (english)
Introduction: Malignant diseases are one of the major causes of mortality in the world, and their incidence rate in the population is increasing. Coumarins are compounds that have an inhibitory effect on certain tumor cells and because of these anti-tumor properties they have potential use in the treatment of malignant diseases. Study aim:To investigate the effect of coumarin derivatives on the growth of normal cells and lymphoma and leukemia cells in vitro. Determine changes in cell cycle and cell death. Materials and Methods: The inhibitory effect of coumarin derivatives (NS-48, NS-51 and A-153) was determined by MTT assay on commercially available human tumor cells (CCRF, K562, Raji and Hut-78) and normal cell line (MDCK1). The alterations of the cell cycle have been monitoredby flow cytometry on the K562 and Raji cell lines. Results: The coumarin derivatives show antiproliferative and cytotoxic effects on human tumor cells. The inhibition depends on the concentration of the compound and the cell line used. Based on the results it can be concluded that all three coumarin derivatives act to inhibit cell growth at a concentration of 1x10-4 M. Conclusion: Coumarin derivatives depending on concentrations and type of cell, inhibit cell growth. Cell cycle and apoptosis changes are also apparent.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:576015
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Medicine Osijek
Created on2019-01-11 10:51:01