Aim: To determine differences in the quality of life of colorectal cancer patients with and without colostomy bag and differences between men and women. Examinees and methods: The study included 64 respondents with a diagnosis of Colorectal Cancer. Research was carried on at Department of Oncology in Clinical Hospital Osijek. The instrument used was a questionnaire EORTC QLQ-C30 version 3.0. Descriptive and nonparametric statistical data analyses were applied. Results: Significant statistic differences is noticed only in particle „overall health during the past week“ (Mann-Whitney U p=0,035) considering colostomy bag. People who have colostomy bag assess their overall health worse than people who do not have it. However, assessment of the poor health conditions did not reflect on the overall quality of life, respectively particle “quality of life during the past week“ is statistically not significantly different (Mann-Whitney U p=0,492). Significant statistic differences does not exist in assessment of overall health and quality of life during the past week considering gender. Conclusion: Patients with colostomy bag perceived their own overall health worse than people who do not have it, but quality of life is not different. Assessment of overall health and quality of life considering gender is not significant statistic different, in other words it does not differ.