OBJECTIVE: The objectives of the study were to examine the frequency of the microorganisms in the conjunctiva and to determine their distribution by age. STUDY DESIGN: cross-sectional study PARTICIPANTS AND METHODS: The study included 682 swabs of patients of both genders and all age groups treated at the Department of Ophthalmology at Clinical Hospital Center Osijek from 2013 to 2017. There were no exclusion criteria. Swabs of conjunctiva and cultures of microorganisms were taken from all participants. RESULTS: This cross-sectional study included 682 conjunctiva swabs of participants. Most of the swabs were taken in 2017, from outpatient participants, and significantly less from hospitalized subjects (χ2 test, P = 0.02). There were fewer swabs taken in 2013 but with no statistically significant difference compared to other years. There was a significant difference in the presence of infection pathogens in relation to the follow-up period (Fisher's exact test, P <0.001), and also a significant difference in the type of infection according to the age of the subject (Fisher's exact test, P <0.001). There was no significant difference in the cause of infection compared to the gender of the participant. CONCLUSION: The most common isolated microorganisms of eye infection were Streptococcus pneumoniae, Staphylococcus aureus, coagulase negative staphylococci and Haemophilus influenzae. Due to age, there was a difference in the type of infection causer.