Objectives: The aim of this study was to determine the correlation between cold ischaemia time and delayed graft function. Furthermore, the aim was to compare patients' characteristics and graft functions between two studied groups, as well as to study the correlation between cold ischaemia time and acute tubular necrosis. Study design: Retrospective cohort study Patients and methods: This study included 42 patients with kidney transplant (52% male, 48% female). Age median was 55 years. All patients were on dialysis prior to the transplantation. All transplants are from deceased donors with diagnosed brain death. Patients were divided into two groups depending on delayed graft function. Results: Median of cold ischaemia time is 872.5 min (14.5 hours). There is no significant statistical correlation between cold ischaemia time and delayed graft function. 24% of patients had delayed graft function. There is no difference in sex, age or HLA mismatch between the two groups. Body mass index is significantly higher in patients with delayed graft function. There is no statistically significant difference between resistance index, creatinine levels and creatinine clearance in the first postoperative medical report. In the second report there are significantly higher creatinine levels, higher resistance index and lower creatinine clearance in patients with delayed graft function. HLA mismatch is not significantly connected with delayed graft function. There is no significant correlation between acute tubular necrosis and cold ischaemia time. Conclusion: Connection between cold ischaemia time and delayed graft function was not determined. The study did not confirm the correlation of cold ischaemia time and acute tubular necrosis. Patients with delayed graft function had significantly worse kidney function when released from the hospital care. Results of this study are in correlation with most referenced works in the field of kidney transplantation.