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undergraduate thesis
Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. urn:nbn:hr:152:762130
Main, Ena
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Main, E. (2020). Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:762130

Main, Ena. "Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:762130

Main, Ena. "Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:762130

Main, E. (2020). 'Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 26 March 2023, https://urn.nsk.hr/urn:nbn:hr:152:762130

Main E. Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2023 March 26] Available at: https://urn.nsk.hr/urn:nbn:hr:152:762130

E. Main, "Utjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:762130

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Metadata
TitleUtjecaj hiperbarične oksigenacije na proteinski izražaj TRP ionskih kanala kod zdravih Spargue-Dawley štakora
Title (english)Effect of hyperbaric oxygenation on protein expression of TRP ion channels in healthy Spargue-Dawley rats
AuthorEna Main
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Utvrditi promjenu proteinskog izražaja TRPV4 proteina u TRP ionskim kanalima izoliranih krvnih žila mozga muških Sprague – Dawley štakora podvrgnutih akutnoj i intermitentnoj terapiji hiperbaričnim kisikom, korištenjem metode Western blot. Nacrt studije: Eksperimentalna studija na pokusnim laboratorijskim životinjama. Ispitanici i metode: Zdravi Sprague – Dawley štakori (n=18) muškog spola u dobi od 9 do 11 tjedana starosti nasumce su razvrstani u tri skupine: CTRL skupina (zdravi netretirani štakori, n=6), A – HBO2 skupina - štakori izloženi akutnoj hiperbaričnoj oksigenaciji (štakori tretirani jednom HBO2 u barokomori te žrtvovani odmah nakon terapije, n=6) i 4D – HBO2 skupina - štakori izloženi intermitentnoj hiperbaričnoj oksigenaciji (štakori izloženi hiperbaričnoj oksigenaciji četiri dana uzastopno te žrtvovani peti dan, n=6). Postupku uzorkovanja prethodilo je vaganje i anesteziranje štakora. Nakon toga izolirane su krvne žile mozga radi određivanja razine proteinskog izražaja KCNMB1 i TRPV4 proteina Western blot metodom. Rezultati: Nije utvrđena statistički značajna razlika u tjelesnoj masi između ispitivanih skupina (p = 0.9421). Proteinski izražaj KCNMB1 i TRPV4 proteina značajno je povećan u skupini 4D–HBO2 u odnosu na proteinski izražaj istih proteina kod CTRL i A–HBO₂ skupine. Zaključak: Intermitentna primjena hiperbarične oksigenacije kod zdravih životinja dovodi do značajno povećanog proteinskog izražaja KCNMB1 i TRPV4 proteina u krvnim žilama mozga Sprague-Dawley štakora.
Abstract (english)
Aim: The main goal of this study was to determine the expression of the TRPV4 protein in brain blood vessels of healthy Sprague-Dawley rats exposed to acute and intermittent hyperbaric oxygenation (HBO₂). Study design: Experimental study on laboratory animals (Sprague – Dawley rats) Materials and methods: 9 – 11 weeks old healthy male Sprague-Dawley rats (n=18) were randomly assigned to three groups: CTRL group (intact animals, n=6), A – HBO2 group - rats that underwent single (acute) hyperbaric oxygenation treatment (n=6) and 4D – HBO2 group - rats underwent intermittent hyperbaric oxygenation (4 consecutive HBO2 treatments for 4 days in a row, n=6). Prior to sampling the animals were weighed and anaesthetized. After that brain blood vessels were isolated in order to determine the protein expression of the KCNMB1 and TRPV4 proteins using the Western Blot method. Results: There was not statistically significant difference in body mass between the experimental groups (p = 0.9421). Protein expression of KCNMB1 and TRPV4 proteins was significantly increased in the 4D – HBO2 group compared to the protein expression of the same proteins in the CTRL and A – HBO₂ groups. Conclusion: Intermittent exposure of healthy Sprague-Dawley rats to hyperbaric oxygenation leads to significantly increased KCNMB1 and TRPV4 protein expressions in the brain blood vessels of Sprague-Dawley rat.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:762130
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Medicine Osijek
Created on2020-12-15 14:08:52