prikaz prve stranice dokumenta Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta
  Download
PDF 643.22 KB
master's thesis
Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. urn:nbn:hr:152:257254
Krivić, Doris
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Cite this document

Krivić, D. (2019). Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:257254

Krivić, Doris. "Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:257254

Krivić, Doris. "Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:257254

Krivić, D. (2019). 'Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:152:257254

Krivić D. Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:257254

D. Krivić, "Usporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:257254

Please login to the repository to save this object to your list.
Metadata
TitleUsporedba metode fluorescentne protočne citometrije i svjetlosne mikroskopskopije u analizi mokraćnog sedimenta
Title (english)Comparison of fluorescence flow cytometry and microscopic examination in urine sediment analysis
AuthorDoris Krivić
MentorVatroslav Šerić (mentor)
Committee memberLjubica Glavaš-Obrovac (predsjednik povjerenstva)
Committee memberSanja Mandić (član povjerenstva)
Committee memberVatroslav Šerić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Usporedba fluorescentne protočne citometrije i svjetlosne mikroskopije u analizi sedimenta mokraće vrlo je bitna jer će omogućiti uvođenje najsuvremenije metode u svakodnevnu kliničku praksu, što rezultira bržom i točnijom analizom sedimenta mokraće. Cilj istraživanja: Cilj je rada usporediti nalaze mokraćnog sedimenta dobivene metodom fluorescentne protočne citometrije s nalazima dobivenim svjetlosnom mikroskopijom. Nacrt istraživanja: Presječno istraživanje Ispitanici i metode: U provedenom istraživanju analizirani su nasumični uzorci mokraće pacijenata KBC-a Osijek. Ukupno je obrađeno 150 uzoraka. U uzorcima mokraće od 150 pacijenata provedena je analiza sedimenta mokraće metodom fluorescentne protočne citometrije na uređaju Sysmex UF-4000 (Siemens) te standardnom metodom svjetlosne mikroskopije. Rezultati: Analizom sedimenta mokraće svjetlosnim mikroskopom i fluorescentnom protočnom citometrijom te njihovom međusobnom usporedbom, vidljivo je značajno odstupanje od međusobnih rezultata. Dokazana je korelacija (P < 0,001) s koeficijentom korelacije (r = 0,64; r = 079; r = 0,52; r = 0,95; r = 0,85) i linearnost (P = 0,19; P = 0,03; P = 0,01; P≤0,01; P≤0,01) između metode fluorescentne protočne citometrije i svjetlosne mikroskopije. Zaključak: Provedbom kliničke usporedbe uređaja Sysmex UF-4000 i svjetlosnog mikroskopa potvrđeno je kako postoje značajna odstupanja u rezultatima analize eritrocita, leukocita, pločastog epitela, hijalinih cilindara i bakterija u sedimentu mokraće.
Abstract (english)
Introduction: Comparison of fluorescent flow cytometry and light microscopy in urine sediment analysis is very important, as it will allow the introduction of a state-of-the-art method into daily clinical practice, resulting in faster and more accurate analysis of urine sediment. Objectives: The aim of the study is to compare the findings of urinary sediment obtained by fluorescent flow cytometry method with the findings obtained by light microscopy. Research Design: Cross-sectional study Subjects and methods: Randomized urine specimens of KBC Osijek patients were analyzed in this study. A total of 150 samples were processed. Urine sediment analysis by fluorescence flow cytometry on the device Sysmex UF 4000 (Siemens) and standard light microscopy were performed on 150 urine samples from the same number of patients. Results: The comparison of the analysis of urine sediment by light microscope and fluorescent flow cytometry method shows a significant deviation from the mutual results. Correlation (P<0.001) with correlation coefficient (r=0.64; r=079; r=0.52; r=0.95; r=0.85) and linearity (P=0.19; P=0.03; P=0.01; P≤0.01; P≤0.01) are proven between the fluorescence flow cytometry and light microscopy. Conclusion: Clinical comparison of Sysmex UF-4000 (Siemens) and light microscope confirmed there are significant discrepancies in the results of urine sediment analysis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:257254
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Medicine Osijek
Created on2021-05-19 08:23:21