The enzyme superoxide dismutase (SOD) defends cells against oxidative damage caused by reactive oxygen species (ROS). SOD is a metalloprotein that belongs to the group of oxidoreductases, and serves to regulate the concentration of ROS within the organism. Considering the metal cofactors present in the active site, SOD is classified into 4 types: Cu, Zn-SOD, Fe-SOD, Mn-SOD, and Ni-SOD. Although its main role is to defend against ROS, the enzyme itself has certain limitations, therefore mimetics (synthetic compounds that mimic its action) are used as a replacement. They are primarily investigated for therapeutic purposes due to their antioxidant activity, smaller size, longer half-life and similarity in action to the native enzyme. Several methods have been developed to determine the mimetic activity of SOD zinc complexes. This thesis compares methods for determining the SOD mimetic activity of zinc complex \( Zn_{1}L_{1}\). Three non-enzymatic methods were used, two of which are based on alkaline DMSO as a source of superoxide anions, and one which uses photoreduction of the riboflavin-methionine system to generate superoxide anions. Method 1 did not determine the SOD activity of the test compound, while Method 2 determined an \( IC_{50}\) of 117 μM. In the case of riboflavin photoreduction methods, the \( IC_{50}\) value is 228 μM, from which it can be concluded that this method is less sensitive and less suitable for determining the SOD mimetic activity of the test compound.