master's thesis
Determination of cations in natural waters using electrophoresis on microchip with C4D detector

Marija Kraševac (2016)
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Metadata
TitleOdređivanje kationa u prirodnim vodama elektroforezom na mikročipu sa C4D detektorom
AuthorMarija Kraševac
Mentor(s)Nikola Sakač (thesis advisor)
Marija Jozanović (thesis advisor)
Abstract
Kapilarna elektroforeza (engl. Capillary electrophoresis, CE) je vrlo učinkovita separacijska tehnika za kvantifikaciju različitih nabijenih specija. Elektroforeza na mikročipu (engl. Microchip electrophoresis, MCE) je minijaturizirana verzija CE. Alkalijske i zemnoalkalijske metale rutinski se prati u različitim uzorcima vode, koji su važni u industriji voda za piće i otpadnih voda industrije. MCE uređaj sa kapacitivno spregnutim beskontaktnim detektorom vodljivosti (engl. CCCCD, engl. Capacitively-Coupled Contactless Conductivity Detection C4D) uspješno je primijenjen za analizu nekoliko kationa, pojedinačno i u smjesi. Separacija je provedena na borosilikatnom mikročipu, duljine separacijskog kanala 4 mm. Kao pomoćni elektrolit (engl. background electrolyte, BGE) korištena je 0,5 M octena kiselina, pH = 2,5. Primijenjeni napon injektiranja iznosio je +0,7 kV, a napon separacije +1,4 kV. Istraživan je utjecaj sljedećih parametara na učinkovitost separacije: vrijeme injektiranja, napon injektiranja, vrijeme i napon separiranja. Ustanovljen je linearni odziv za svaki pojedini ispitivani kation, pojedinačno i u smjesi pomoću linearne regresije. Metoda je primijenjena kod određivanja kationa u vodi iz slavine, flaširanoj vodi i u kišnici. Metodički dio rada sadrži pripremu za nastavnu jedinicu Osnovna svojstva tekućina. Nastavni sat je zamišljen kao kombinacija frontalnog i grupnog rada sa pokusima. Grupnim radom nastoji se osamostaliti učenike, a pokusima im se nastoji približiti gradivo te potiče na razmišljanje i donošenje zaključaka.
Keywordscations water natural waters C4D detector microchip electrophoresis microfluidic
Parallel title (English)Determination of cations in natural waters using electrophoresis on microchip with C4D detector
Committee MembersMilan Sak-Bosnar (committee chairperson)
Nikola Sakač (committee member)
Ivan Vicković (committee member)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Chemistry
Study programme typeuniversity
Study levelgraduate
Study programmeChemistry
Academic title abbreviationmag.educ.chem.
Genremaster's thesis
Language Croatian
Defense date2016-09-29
Parallel abstract (English)
Capillary electrophoresis (CE) is a highly efficient separation technique for quantitation of different charged compounds. Electrophoresis on microchip (MCE) is a miniaturized version of the CE.Alkali and earthalkali metals were routinely monitored in a variety of aqueous samples important to several industries, such as drinking and waste water industries.In this investigations a MCE device with capacitively coupled contactless conductivity detection (engl. Capacitively-Coupled Contactless Conductivity Detection, CCCCD, C4D) has been successfully applied for analysis of several cations, individually and in mixture. The separation was carried out on a borosilicate microchip with the separation channel length of 4 mm. Acetic acid solution of the concentration 0.5 M has been used as a background electrolyte (pH = 2.5). The applied injection voltage was +0.7 kV and the separation voltage was +1.4 kV. The influence of the following parameters on the separation ability was investigated: injection time, injection voltage, separation time and separation voltage. The linear response region for each particular cation investigated, both single and in a mixture, has been determined using linear regression. The method was applied to determine cations in tap water, mineral water and rain water.In the teaching part of the thesis it is proposed a preparation lecture called The basic properties of liquids. Lesson is designed as a combination of frontal lectures and students group work with experiments. Aim of the group work is to achieve the independence of students, and experiments are helpfull for thinking about teaching materials and to make conclusions.
Parallel keywords (Croatian)kationi voda prirodne vode C4D detektor elektroforeza mikročip mikrofluidika
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:182:139931
CommitterSanda Hasenay