Three-of-heaven (Ailanthus altissima (Mill.) Swingle) is considered as one of the most invasive plant species worldwide in rural and urban environment. Although tree-of-heaven is commonly observed throughout the city of Osijek (Croatia), its prevalence and frequency has not been documented. This study has been conducted from May to September 2022 in the streets of Osijek to determine the distribution, abundance and competitive advantage of this species. A survey was performed by sampling randomly selected 20 quadrats in each city district, having together 140 phytocoenological records, and presence-absence of tree-of heaven was recorded in each plot. Where present at urban habitats, the number of threes were recorded and categorized approximately by the diameter at breast height (dbh). Habitats were distinguished as follows: alleys, abandoned private areas, public areas, ruderal sites, cracked walls and road edges. Risk assessment analysis were prepared by combining quantitative (number of trees per plots) and qualitative (four categories: biological characteristics and dispersal abilities, ecological amplitude and distribution, ecological impact, and difficulty to control) data. There was e recorded a total of 189 tree-of-heaven, occurring both as solitary plants or in developed stands. The habitat types with the highest rate of tree-of-heaven are abandoned private areas (34%) followed by public areas (31%). Out of the total, 55% of the recorded tree-of heaven are young individuals belonging to the very small and small diameter classes, and are mainly present on abandoned private and public areas. Ecological impact and biological characteristics are primary course of its landscape spread.