Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)

Mataković, Lavinija

prikaz prve stranice dokumenta Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)

master's thesis

Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)

Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2012. urn:nbn:hr:181:452114

Mataković, Lavinija

Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

APA 6th Edition

Mataković, L. (2012). Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:452114

MLA 8th Edition

Mataković, Lavinija. "Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2012. https://urn.nsk.hr/urn:nbn:hr:181:452114

Chicago 17th Edition

Harvard

Mataković, L. (2012). 'Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:181:452114

Vancouver

Mataković L. Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2012 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:181:452114

IEEE

L. Mataković, "Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:181:452114

Cite this item: https://urn.nsk.hr/urn:nbn:hr:181:452114

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Metadata

Title	Učinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)
Title (english)	Influence of ß-cryptogein transgene on catalase and guaiacol-peroxidase activity in Coleus blumei hairy roots
Author	Lavinija Mataković
Mentor	Elizabeta Has Schon (mentor)
Committee member	Elizabeta Has Schon (predsjednik povjerenstva)
Committee member	Ljiljana Krstin (član povjerenstva)
Committee member	Ivna Štolfa Čamagajevac (član povjerenstva)
Committee member	Janja Horvatić (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Department of biology Osijek
Defense date and country	2012-09-28, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Svojstvo biotičkih elicitora da potiču obrambene odgovore u biljkama koristi se u in vitro kulturi kao strategija za povećanu proizvodnju komercijalno važnih spojeva. Elicitori se također koriste i u svrhu poboljšanja otpornosti biljke na patogene i neke okolišne stresove. Beta-kriptogein je proteinski elicitor koji izlučuje fitopatogena oomiceta Phythophthora cryptogea, koji kod biljaka izaziva hipersenzitivni odgovor i sustavno stjecanje otpornosti protiv nekih patogena. Cilj ovog istraživanja je kontroliranom sintezom kriptogeina u biljci oponašati napad patogenom, te na taj način poboljšati antioksidativni status biljke. U tu svrhu je transgeno korijenje ukrasne koprive (Coleus blumei), u kojem se sintetčki gen crypt nalazi pod kontrolom inducibilnog alkoholnog promotora, tretirano etanolom kako bi se inducirala sinteza ß-kriptogeina. Kao pokazatelji oksidativnog stresa mjerene su količine vodikova peroksida (H2O2) i produkata lipidne peroksidacije (TBARS). Antioksidativni staus je određen mjerenjem aktivnosti katalaze i gvajakol-peroksidaze. Inducibilna ekspresiju gena crypt je povećala aktivnosti antioksidativnih enzima (katalaze i gvajakol-peroksidaze) u kosmatom korijenju ukrasne koprive. Razine H2O2 i TBARS-a su pokazale odusutnost oksidativnog stresa. Povećani antioksidativni status mogao bi poboljšati otpornost biljaka protiv patogena i drugih okolišnih stresova.
Abstract (english)	The property of biotic elicitors to induce plant defence response was used as an strategy for enhancing production of commercially important compounds in plant in vitro cultures. Also, elicitors have been used to improve plant resistance to pathogens and to some environmental stresses. Proteinaceus elicitor secreted by phytophatogenic oomycete Phythophthora cryptogea, ß-cryptogein, induces hypersensitive response and systemic acquired resistance in plants against some pathogens. The aim of this thesis was to mimic pathogen attack by using controlled synthesis of cryptogein, and thus improve antioxidant status of the plant. In order toinduce synthesis of the ß-cryptogein Coleus blumei hairy roots harboring synthetic crypt gene were generated under the control of alcohol-inducible promoter after ethanol treatment. As an indicator of the oxidative stress hydrogen peroxide (H2O2) and lipid peroxidation product (TBARS) contents were estimated. Antioxidative status was determined by measuring catalase and guiacol peroxidase activities. Induced expression of crypt gene caused increase of antioxidant enzymes activities (GPOD and CAT) in Coleus blumei hairy roots. Contents ofH2O2 and TBARS confirmed the absence of oxidative stress. Increased antioxidative status could provide enhance resistance against pathogens and other environmental stresses.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:181:452114
Study programme	Title: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology Course: Master of Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Department of biology, Josip Juraj Strossmayer University of Osijek
Created on	2017-01-24 11:53:32