No public access
master's thesis
ROLE OF ADIPOKINETIC HORMONES IN STRESS ELICITED BYINSECTICIDES: TRIBOLIUM CASTANEUM MODEL

Ivana Plavšin (2015)
Josip Juraj Strossmayer University of Osijek
Department of biology
Metadata
TitleULOGA ADIPOKINETIČKIH HORMONA U STRESU IZAZVANOMINSEKTICIDNIM TRETMANOM KOD VRSTE TRIBOLIUM CASTANEUM
AuthorIvana Plavšin
Mentor(s)Branimir K.Hackenberger (thesis advisor)
Abstract
Adipokinetički hormoni (AKH) su skupina neuropeptida prisutnih kod kukaca koji se pojačano luče u uvjetima stresa kako bi potakli odgovarajuće obrambene reakcije te održali homeostazu organizma. Primarno su uključeni u kontrolu metabolizma, al itakođer aktiviraju i antioksidativni sustav organizma. U ovom radu kao modelni organizam korištena je vrsta Tribolium castaneum, a kao stresor dva insekticida(pirimifos-metil i deltametrin). Kukci su izlagani samo insekticidima te insekticidima u kotretmanu s AKH. Nakon tretmana, mjeren je mortalitet, brzina metabolizma kukaca kao i aktivnost biomarkera oksidativnog stresa. Kako bi se istražila uloga AKH u stresnim uvjetima, pripremljen je i soj AKH-deficijentnih kukaca pomoću RNAi tehnologije te je isti podvrgnut jednakim tretmanima kao i normalni soj.
KeywordsTribolium castaneum insecticides adipokinetic hormones RNAitechnology oxidative stress mortality
Parallel title (English)ROLE OF ADIPOKINETIC HORMONES IN STRESS ELICITED BYINSECTICIDES: TRIBOLIUM CASTANEUM MODEL
Committee MembersDavorka Hackenberger Kutuzović (committee chairperson)
Branimir K.Hackenberger (committee member)
Goran Palijan (committee member)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelgraduate
Study programmeMaster of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Study specializationMaster of Biology
Academic title abbreviationmag.biol.
Genremaster's thesis
Language Croatian
Defense date2015-05-07
Parallel abstract (English)
Adipokinetic hormones (AKHs) are insect neuropeptides which major function is to stimulate immune response and to maintain homeostasis in living organism. (AKHs) are primary included in metabolism control, but they also activate insect's antioxidative system. In the present study, Tribolium castaneum (red flour beetle) was used as a model species, and two insecticides as stressors (pirimiphos-methyl and deltamethrin).Insects were exposed to insecticides alone and to insecticides in combination with AKH.Mortality, metabolism rate, and activity of oxidative stress biomarkers were measuredafter the treatment. AKH-deficient beetles were prepared using RNAi technology in order to investigate role of AKHs under stress conditions. They were exposed to the same conditions as normal beetle strain.
Parallel keywords (Croatian)Tribolium castaneum insekticidi adipokinetički hormoni RNAitehnologija oksidativni stres mortalitet
Resource typetext
Access conditionNo public access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:181:581141
CommitterVesna Radman Meic