prikaz prve stranice dokumenta Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom
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master's thesis
Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. urn:nbn:hr:181:930101
Mihajlović, Lovro
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Mihajlović, L. (2022). Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:930101

Mihajlović, Lovro. "Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. https://urn.nsk.hr/urn:nbn:hr:181:930101

Mihajlović, Lovro. "Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. https://urn.nsk.hr/urn:nbn:hr:181:930101

Mihajlović, L. (2022). 'Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:181:930101

Mihajlović L. Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2022 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:181:930101

L. Mihajlović, "Promjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:181:930101

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Metadata
TitlePromjene lipidome mijelina mozga B4galant1 miša izazvane progresivnom demijelinizacijom
Title (english)Changes in brain myelin lipidomics of B4galant1 mouse coused by progressive demyelination
AuthorLovro Mihajlović
MentorMarija Heffer (mentor)
MentorDario Mandić (komentor)
MBZ: 367140
Committee memberSenka Blažetić (predsjednik povjerenstva)
Committee memberMarija Heffer (član povjerenstva)
Committee memberIrena Labak (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2022, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Gangliozidi su membranski lipidi koji, svojim razgranatim šećernim lancima, sudjeluju u izgradnji glikokaliksa neurona. Zbog svog vanjskog ugljikohidratnog dijela mogu sudjelovati u interakcijama s raznim molekulama. Primjerice, složeni gangliozidi na membrani aksona ulaze u interakcije s MAG proteinima (engl. myelin-associated glycoprotein) izraženima na mijelinskim ovojnicama oligodendrocita. Na taj se način održavaju povoljne interakcije između aksona i oligodendrocita, što ima izraziti metabolički značaj i osigurava preživljavanje aksona. B4galnt1 KO (engl. knock out) miševi jesu miševi kojima je isključen gen zadužen za ekspresiju enzima koji katalizira nastajanje složenih gangliozida. Umjesto toga, nastaju samo jednostavni gangliozidi koji ne ulaze u interakcije s MAG proteinima, zbog čega takvi miševi u starijoj dobi razvijaju Wallerijansku degeneraciju, demijelinizaciju i poremećaj u motoričkim sposobnostima. U ovom se diplomskom radu s pomoću MALDI-TOFMSI analize analizirala bijela tvar malog mozga B4galnt1 KO miševa i miševa divljeg tipa te je otkriveno nekoliko metabolita koji pokazuju niže razine u B4galnt1 KO miševima i koji su od velike važnosti za mozak te se povezuju s neurodegenerativnim bolestima. Neki od tih metabolita su gvanozinski nukleotidi, citidin trifosfat, UDPaktivirani N-acetilirani aminošećeri i fumarikarnitin.
Abstract (english)
Gangliosides are membrane lipids that participate in the construction of the neuron glycocalyx with their branched sugar chains. Due to this external carbohydrate part, they can participate in interactions with various molecules. For example, complex gangliosides on the axon membrane enter into interactions with MAG (myelin-associated glycoprotein) expressed on the oligodendrocyte myelin sheaths. In this way, favorable interactions between axons and oligodendrocytes are maintained, which has a distinct metabolic significance and ensures the axon survival. B4galnt1 KO (knock out) mice are mice in which the gene responsible for the expression of the enzyme that catalyzes the formation of complex gangliosides has been turned off. Instead, only simple gangliosides are produced that do not interact with MAG proteins, which is why such mice develop Wallerian degeneration, demyelination, and impaired motor skills in old age. In this thesis, white matter of the cerebellum of B4galnt1 KO mice and wild-type mice was analyzed using MALDI-TOF-MSI analysis, and several metabolites were detected that show lower levels in B4galnt1 KO mice and that are of great importance for the brain due to their association with the neurodegenerative diseases. Some of those metabolites are guanosine nucleotides, cytidine triphosphate, UDP-activated N-acetylated aminosugars and fumarycarnitine.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:930101
Study programmeTitle: Master of Science Graduate University Study Programme in Biology and Chemistry; specializations in: Master Education In Chemistry and Biology
Course: Master Education In Chemistry and Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
Terms of useLicence In copyright icon
RepositoryRepository of Department of biology, Josip Juraj Strossmayer University of Osijek
Created on2022-11-02 11:49:45