undergraduate thesis
Selenium -antioxidant or prooxidant?

Rebeka Bošnjaković (2016)
Sveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
Metadata
TitleSelen - antioksidans ili prooksidans?
AuthorRebeka Bošnjaković
Mentor(s)Ivna Štolfa Čamagajevac (thesis advisor)
Abstract
Selen je u tragovima esencijalan mikronutrijent i iznimno je koristan za čovjeka i životinje, no njegova esencijalnost nije dokazana za više biljke. Specifični fiziološki mehanizmi, koji su zaslužni za njegovo pozitivno djelovanje u antioksidacijskoj obrani biljaka još nisu u potpunosti razjašnjeni. Selen ima dvojako djelovanje na biljke; u malim koncentracijama djeluje kao antioksidans, dok u velikim djeluje kao prooksidans. Cilj ovog rada bio je utvrditi kako različiti oblici i koncentracije Se utječu na antioksidacijski sustav, odnosno aktivnosti glutation-reduktaze i katalaze u izdancima pšenice (Triticum aestivum L.) sorte Divana. Pšenica je tretirana trima različitim koncentracijama dva oblika Se (selenit i selenat). Sve koncentracije oba oblika selena nisu aktivirale katalazu.S druge strane, povećanje aktivnosti glutation-reduktaze uzrokovale su samo dvije veće koncentracije selenata, dok je taj efekt izostao kod tretmana selenitom što se može povezati s učinkovitijom mobilnošću selenata u nadzmne dijelove biljke.Dobiveni rezultati mogu poslužiti kao osnova za daljnja istraživanja o učincima viših koncentracija selena na različite komponente antioksidativnog sustava.
Keywordsselenium oxidative stress glutathione reductase catalase Triticum aestivum
Parallel title (English)Selenium -antioxidant or prooxidant?
Committee MembersIvna Štolfa Čamagajevac (committee chairperson)
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelundergraduate
Study programmeUndergraduate University Study Programme in Biology (Bachelor of Science)
Academic title abbreviationuniv.bacc.biol.
Genreundergraduate thesis
Language Croatian
Defense date2016-09-20
Parallel abstract (English)
As a trace elementselenium is an essential micronutrinet and has important benefits for human and animals, but its essentiality has not been proven yet for higher plants. The specific physiological mechanisms that are responsible for positive effects in plants, primarily in antioxidant defense, have not yet been clarified. Selenium has dual effect on plants, in low concentrations acts as an antioxidant, while in higher concentrations acts as a pro-oxidant. The aim of this study was to determine how different forms and concentrations of selenium influence the antioxidant system, especiallyactivities of glutathione reductase and catalase in the shoots of wheat (Triticum aestivum L.) cv. Divana. Wheat was treated with three different concentrations of two forms of Se (selenite and selenate). All concentrations of both selenium forms did not activate catalase. On the other hand, only the two highest selenate concentrations caused increase in glutathione reductase activity, while selenite did not have such an effect what can be connected with more efficient mobility of selenate in upper parts of plants. Obtained results can serve as a basis for fruther research of the effects of higher selenium concentrations on the various antioxidant systemcomponents.
Parallel keywords (Croatian)selen oksidacijski stres glutation-reduktaza katalaza Triticum aestivum
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:181:256751
CommitterVesna Radman Meic